LOCUS       40924_44569        5078 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pTY262 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5078)
  AUTHORS   Yamaguchi H.
  TITLE     Cloning vector pTY262
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5078)
  AUTHORS   Yamaguchi H, Ohyama A, Nunome T, Miyatake K, Fukuoka H.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; 
            Kusawa 360, Tsu, Mie 514-2392, Japan
REFERENCE   3  (bases 1 to 5078)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5078)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (18-JUL-2012) Contact:Hirotaka Yamaguchi NARO, NIVTS; Kusawa 360, 
            Tsu, Mie 514-2392, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5078
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      4..459
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      complement(679..930)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    961..1992
                     /gene="TUA6"
                     /label=first intron of tomato tubulin gene
                     /note="first intron of tomato tubulin gene"
     gene            961..1992
                     /gene="TUA6"
                     /label=TUA6
     promoter        complement(2024..2369)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     promoter        complement(2890..2908)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2929..2945)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2953..2969)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2977..3007)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3022..3043)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(3331..3919)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4093..4950)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4951..5055)
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggaggcgcg
      661 ccgcggccgc tcgggcccga tctagtaaca tagatgacac cgcgcgcgat aatttatcct
      721 agtttgcgcg ctatattttg ttttctatcg cgtattaaat gtataattgc gggactctaa
      781 tcataaaaac ccatctcata aataacgtca tgcattacat gttaattatt acatgcttaa
      841 cgtaattcaa cagaaattat atgataatca tcgcaagacc ggcaacagga ttcaatctta
      901 agaaacttta ttgccaaatg tttgaacgaa ttcgagctcg tcgacggtat cgataagctt
      961 ttgggagaaa taacaagatc atcagcaatg cctctaaaac aggttcttag caacagatac
     1021 aattctaatg gataacatgt ttttcatata tccaaataat ttgagtaatg tcatagaatc
     1081 tcacaaaaga acctaactat tggatacatt agctaataaa atttcaaagt ataataacta
     1141 ccattcattc acaataaatc atacatgtat tattcagttc tgcgcccatc tctatggtac
     1201 actagtttta atatagcatt gaagatgatc catctaatgt agacatatac acacataaat
     1261 ttctatacat ctatactatg gacatccatc cgatctaatg gacatgaaat gaatctatcc
     1321 aaaaaattcg actttcaaat cattttcaca tcatattatc caaacagatc catgtaaaaa
     1381 ctttcacatt tgaacagatt caatcaaata ttgatgttac tcaaacagat atatgcatca
     1441 atatatctat caaaaagagt ggattcaaat gtttgtgacg tcaatcaaat gttatttttt
     1501 actactcaaa cagatttatg catcaatata tctatcaaaa agagtggatt caaatgtttg
     1561 tgacgtctat caaatgttat tttttactac tcaaacgatg tgcattcaaa tcattttgat
     1621 atcaccatca ttcacacaga tctatatcat tacatctgca tttgaaaaga gatttatcat
     1681 aaagcaaatt gatctaccta aatatatgtg gatcaaatgt tctttcaaaa tcaacgggac
     1741 tcaaaacaaa taattcaaac agatctatac aagaacgctt cgcatttgaa gagatcaatg
     1801 cacaacatta tatatcattc aaacagattg aagaaacaca tagatctatg tataaaatac
     1861 aaattcaaat aaattttaaa tcaaaggaac gtaaattcag agattcaaac agatctacac
     1921 aaaaattcaa atatacaaaa acaattagaa atttaagttt gaaaagataa agaaatggtg
     1981 aaaaatacaa acctgcagcc cgggggatcc ctcgagggta ccgtgttctc tccaaatgaa
     2041 atgaacttcc ttatatagag gaagggtctt gcgaaggata gtgggattgt gcgtcatccc
     2101 ttacgtcagt ggagatatca catcaatcca cttgctttga agacgtggtt ggaacgtctt
     2161 ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg tcggcagagg
     2221 catcttcaac gatggccttt cctttatcgc aatgatggca tttgtaggag ccaccttcct
     2281 tttccactat cttcacaata aagtgacaga tagctgggca atggaatccg aggaggtttc
     2341 cggatattac cctttgttga aaagtctcaa ttgccctttg gtcttctgag actgtatctt
     2401 tgatattttt ggagtagaca agtgtgtcgt gctccaccat gttgacgaag attttcttct
     2461 tgtcattgag tcgtaagaga ctctgtatga actgttcgcc agtctttacg gcgagttctg
     2521 ttaggtcctc tatttgaatc tttgactcca tggcctttga ttcagtggga actacctttt
     2581 tagagactcc aatctctatt acttgccttg gtttgtgaag caagccttga atcgtccata
     2641 ctggaatagt acttctgatc ttgagaaata tatctttctc tgtgttcttg atgcagttag
     2701 tcctgaatct tttgactgca tctttaacct tcttgggaag gtatttgatt tcctggagat
     2761 tattgctcgg gtagatcgtc ttgatgagac ctgctgcgta agcctctcta accatctgtg
     2821 ggttagcatt ctttctgaaa ttgaaaaggc taatctagag cggccgcggc gcgcctccag
     2881 cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt catagctgtt
     2941 tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa
     3001 gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact
     3061 gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc
     3121 ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg
     3181 ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
     3241 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
     3301 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
     3361 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
     3421 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
     3481 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
     3541 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
     3601 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
     3661 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
     3721 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
     3781 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
     3841 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
     3901 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
     3961 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
     4021 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
     4081 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     4141 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     4201 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     4261 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     4321 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     4381 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     4441 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     4501 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     4561 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     4621 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     4681 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     4741 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     4801 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     4861 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     4921 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     4981 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     5041 aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
